A rapid and efficient electroporation method for transformation of Halomonas sp. O-1
نویسندگان
چکیده
منابع مشابه
A REPORT OF RAPID AND EFFICIENT METHOD FOR MITOCHONDRIAL DNA
Based on the similarity of prokaryotic DNA to that of mitochondrial DNA, a rapid and efficient method for DNA purification from mitochondrial extracts obtained from different sources, was carried out based on the modification method described by Marmur (1983) which resulted in a high degree of purity (A260/A280=2). In addition we have applied the same technique for the first time to purify...
متن کاملa report of rapid and efficient method for mitochondrial dna
based on the similarity of prokaryotic dna to that of mitochondrial dna, a rapid and efficient method for dna purification from mitochondrial extracts obtained from different sources, was carried out based on the modification method described by marmur (1983) which resulted in a high degree of purity (a260/a280=2). in addition we have applied the same technique for the first time to purify the ...
متن کاملIsolation of moderately halophilic bacteria from Tunisian soil samples and transformation by electroporation using Escherichia coli-Halomonas sp. shuttle vector
Halophilic and salt-resistant bacteria were isolated from soil samples collected from Tunisia. Among them, strain 21a grew at NaCl concentration ranging 1-15%, optimum at 3%, showing that it is moderately halophilic bacteria. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain 21a was closely related to Halomonas aquamarina and H. venusta with sequence similarities of 9...
متن کاملA Simple and Rapid Method of Transformation of Streptomyces rimosus R6 and Other Streptomycetes by Electroporation.
Usually plasmid DNA is introduced into Streptomyces strains by polyethylene glycol-mediated transformation of protoplasts. However, many Streptomyces strains are only poorly or not at all transformable via protoplasts. Therefore, we have optimized the parameters critical for the application of electrotransformation of plasmid DNA into Streptomyces species. The most critical parameters evaluated...
متن کاملHalomonas gomseomensis sp. nov., Halomonas janggokensis sp. nov., Halomonas salaria sp. nov. and Halomonas denitrificans sp. nov., moderately halophilic bacteria isolated from saline water.
A total of 34 Halomonas strains were isolated from saline water in Anmyeondo, Korea. Ten of these strains, considered to belong to novel species, were subjected to a polyphasic taxonomic investigation. The strains were Gram-negative, moderately halophilic, motile and non-spore-forming rods that contained Q-9 as the predominant ubiquinone and C(18 : 1)omega7c, C(16 : 0) and either summed feature...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Microbiological Methods
سال: 2016
ISSN: 0167-7012
DOI: 10.1016/j.mimet.2016.08.009